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Histone deacetylases including SIRT1, interact with STAT3 and negatively regulate its transcriptional activity by deacetylating critical lysine residues (Nie et al., 2009)
They maintain refrigerator temperatures for 8 to 14 hours depending on the case quality and ambient temperature
LipofectAMINETM 2000 (Invitrogen) according to the manufacturer's protocol
