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First, mitochondrial dysfunction manifests as the accumulation of mtDNA mutations [57, 58], disrupted energy metabolism [59], and elevated reactive oxygen species (ROS) production [60], severely affecting the energydependent substantia nigra dopaminergic neurons [61]

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A structured and standardized spreadsheet was used to record the following: study authors, year of publication, abstract, type of model used ( in vivo or in vitro ), parameters evaluated (biomarkers, gene expression, histological alterations, among others), applied methodology, and key findings

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