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Three-chamber testing was used to assess social behavior [39]
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Steroidogenic acute regulatory (StAR) gene expression levels were measured using the comparative Ct (Ct) approach to determine the relative quantitative expression of the StAR gene relative to the control (untreated sample) and then normalized to the -actin reference gene
Studies show MT-II reduces food intake in a dose-dependent manner and leads to sustained body mass reduction and lower adiposity, even after appetite normalizes (5)
