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Description
Two-way analysis of variance (ANOVA) was employed to determine the main effects of age and time, or age and dose
For samples derived from media containing 13 C glucose or 13 C glutamine, methoximation was followed by silylation by adding 80 l of MTBSTFA (with 1% TBDMS, Thermo) and incubation for 60 min at 70 C
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Holding the open ampuole at an angle in one hand, use the other hand to carefully place the needle near the bottom and draw-up the B12